The first report of Choiromyces venosus and a new report of Tuber aestivum from north Hyrcanian forest of Iran

Document Type : Research paper


1 Department of Forestry and forest economy, Faculty of Natural Resources, University of Tehran, Karaj, Iran

2 Department of Plant Protection, Faculty of Agricultural Science and Engineering, College of Agriculture and Nature Resources, University of Tehran, Karaj

3 Department of Ecosystems and Society, Oregon State University, Corvallis, USA


Luxurious truffles have always been among the most popular symbiotic microorganisms, but there have been limited efforts to identify specific truffle species in forest systems around the world. Recent developments in molecular techniques have rapidly improved our ability to identify microorganisms responsible for the formation of truffles with different tree species. In the present study, an attempt was made to identify and describe two species of truffles occurring in deciduous forests of Golestan and Mazandaran provinces of Iran. Choiromyces venosus and Tuber aestivum were identified by a combination of morphological characterization, cytological methods and molecular analysis. Molecular characterization was performed by amplification and sequencing of the internal transcribed spacer regions (ITS) of nuclear rDNA. Based on the ITS sequences, Iranian specimens were placed in the same branch in a clade with T. aestivum and C. venosus. All sequences of C. venosus and T. aestivum, including the Iranian specimens, had an average similarity of 99.5% (the range was from 99 to 100%). Moreover, the diverse forests of Iran are a rich habitat for the ectomycorrhiza of truffle fungi. Due to the wide range of climatic conditions and forest communities in Iran, further studies are needed on the identification, diversity, ecology, phylogeny and economic value of Iranian truffles.

Graphical Abstract

The first report of Choiromyces venosus and a new report of Tuber aestivum from north Hyrcanian forest of Iran


  • Identification of truffle fungi in Iran is as important as in the world because there are the rich diversity forests in Iran but the introducing and investigation on truffles are limited.
  • The first report of Choiromyces venosus and fourth report of Tuber aestivum the edible truffle mushroom in Iran.


Main Subjects

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